The last AP is shown magnified in the inset (right bottom), with the depolarizing pulse below the end of the stimulus artifact (S) is seen as an inflection preceding the unclamped axon spike (AP). Note that the 20 depolarizing pulses evoked 20 AP's without failures. Raw traces of evoked AP's are shown in the bottom panel (red trace). The decay followed a single exponential curve the decay time constant τ (in msec) is indicated on each panel. Moreover, through the histograms and cumulative histograms, intermediary indexing is applied by Midway image. The images already extracted by pseudocolor map are segmented by red-channel conversion for turning into grayscale and 4-level quantization. The raw Ca 2+ responses showed the typical waveform of AP-induced Ca 2+ responses, with a fast rising and slow decay phase. We therefore proposed a novel quantization and indexing algorithm in hand thermography. Presynaptic Ca 2+ responses were evoked by trains of 5, 10 or 20 AP's. This stretch of axon contained one hot spot, indicated by the arrowhead (B2). The area outlined in panel B1 (white rectangle) is shown enlarged in the inset (B2). The recorded MSN is shown in a projection image (B1) made from the full confocal z series of Alexa594 images (50 sections at 1 μm intervals). Dendritic processes were studded with spines, in contrast to the single axonal process, which was free of spines. At 30 min (A2), dendritic labeling showed no significant change, while the axon was now clearly labeled. At 5 min, the dendrites and proximal axon were filled. Projection images (A1) were created from a z series of confocal Alexa594 images. Fluorescence intensity is shown in pseudocolor (ImageJ). Returns true if this image uses a pseudocolor or grayscale LUT, in other words, is this an image that. A single MSN was loaded with OG1 and Alexa594 through the patch pipette and imaged by 2PLSM at 5 and 30 min after entering whole cell mode. ![]() Pseudocolor Images A pseudocolor (or indexed color) image is a single channel gray. Delayed dye diffusion identifies MSN axons. 8 Stacks, Virtual Stacks and Hyperstacks Stacks ImageJ can display.
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